求助:大哥们帮我看看这个翻译可以吗?
[b][color=red][size=4]帮忙修改得好的奖3个威望!参与者一般都有奖励![/size][/color][/b]老板硬要我翻译,可我连英语4级都没过啊。哪位行行好帮我看下,不好的地方帮我修改一下好吗?万分感谢!
取健康鱼精或胸腺适量,绞碎,分离核蛋白和脱氧核蛋白,再解离脱氧核蛋白,通过离心,去除蛋白,进一步纯化脱氧核糖核酸,减压干燥即得。Fetch health milt or thymus right amount , triturate , dissociate nucleoprotein and deoxidation nucleoprotein,and then disaggregate the deoxidation nucleoprotein , through centrifugation , get rid of the albumen, further sublimate DNA, it reduces pressure dry to be.
3、identify
取本品50 mg溶于二苯胺试液(取二苯胺1 g,加冰醋酸100 ml,再加入70-72 %高氯酸10 ml,混匀,临用前加入1.6%乙醛溶液1ml即得)50 ml,摇匀,置于60℃温水浴加热1 h,溶液应显蓝色。Fetch the product to dissolve in 50 ml diphenylamine solutions (fetch diphenylamine 1 g , add glacial acetic acid 100 ml , join again 70-72% of Perchloric acid 10 ml, mix even , Put into 1.6% acetic aldehyde solution 1ml before using ) , shake evenly before using, put and heat 1 h in 60 degrees Centigrade of warm water, the solution should show the blue.
4、external appearance
取样品在非直射阳光条件下进行目测:白色或类白色或淡黄色固体。The product is estimated under not penetrating the sunshine condition directly: White or a kind of white or yellowish solid.
5、 shape
取样品在非直射阳光条件下进行目测:絮状或粉末状物体。The product is estimated under not penetrating the sunshine condition directly: Wad the form or the powder form object with cotton.
6、 purity mensuration
6、1定磷法原理principle of determines the content of phosphorus
本法把DNA中的有机磷转变为无机磷后进行磷含量的测定。磷酸根在酸性溶液中与钼酸铵生成磷钼酸铵,遇还原剂即生成蓝色物质(三氧化钼和五氧化钼的混合物),称之为“molybdenum blue”,用比色法测定样品中磷含量。Carry on the survey of the content of phosphorus after this law changes the organic phosphorus in DNA into the inorganic phosphorus. Phosphoric acid root turn into phosphorus ammonium molybdate with ammonium molybdate among acid solution , meet reducing agent turn into the blue material promptly (oxidize the molybdenum and oxidize the mixture of the molybdenum five times three times), call it " molybdenum blue ", using colorimetry determines the content of phosphorus.
6、2二苯胺法原理
DNA在酸性条件下加热,其嘌呤碱与脱氧核糖间的糖苷键断裂,生成嘌呤碱、脱氧核糖和脱氧嘧啶核苷酸,而α-脱氧核糖在酸性环境中加热脱水生成ω-羟基-γ-酮基戊糖,与二苯胺试剂反应生成蓝色物质,在595nm波长处有最大吸收。DNA在40~400μg范围内,光吸收与DNA的浓度成正比。在反应液中加入少量乙醛,可以提高反应灵敏度。DNA is heated under the acid condition , the glucoside key among its purine alkali and deoxyribose ruptures , it produce purine the alkalies , deoxyribose and deoxidation not sour in nucleoside in pyrimidine,and α- there aren't deoxyribose, with is it turn into getting blue material to react by two aniline reagent, wavelength office have most heavy to absorb in 595nm. DNA is within the range of 4000μg, it is in direct proportion to density of DNA to only absorb. Put into a small amount of acetic aldehyde in reflecting the liquid , can improve the sensitivity of reacting .
7、氯离子检查Chlorine ion
取本品适量,加水制成每1 ml中含1 mg的溶液,取6 ml依法测定(中国药典2000年版二部附录Ⅲ A),与标准氯化钠溶液 6ml制成的对照液比较,不得更浓(1.0%)。
fetch copies of right amount product to check , add water make every 1 ml including 1 mg solution , fetch 6 ml determine in accordance with the law (Chinese pharmacopoeia 2000 editions of two appendix Ⅲ A), with contrasting there aren't liquid sodium chloride not standard solution, no getting denser(1.0%). [b][size=4][color=limegreen]帮忙修改得好的奖3个威望!参与者一般都有奖励!
不过, 楼主自己的翻译有些明显的错误, 最好自己先改正![/color][/size][/b] 谢谢版主帮忙,感激不尽!我的英语实在不行啊,我自己改了2天了。还在网上找了好多工具,可还是搞不定。 由于水平和时间有限,今天就只能弄这么多了,版主说的很有道理,楼主你自己也要努力啊。
取健康鱼精或胸腺适量,绞碎,分离核蛋白和脱氧核蛋白,再解离脱氧核蛋白,通过离心,去除蛋白,进一步纯化脱氧核糖核酸,减压干燥即得。
Fetch some healthy milt or sufficient thymus , pound it to pieces, seperate nucleoprotein from deoxidation nucleoprotein,and then dissociate the deoxidation nucleoprotein , centrifugate , get rid of the albumen, after that sublimate DNA, then depress and dry it. 专业不对口,不熟,帮补上忙! 谢谢heizilxg,我自己也会再改的,也谢谢带刺水母 专业不对口 这样的形式非常值得鼓励!以后要多开展这方面的交流!一次问题不要太多,针对一两个问题,如sam斑竹说的,参与者有奖!提出好问题的也有奖!
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