[求助]DNAstar序列拼接
我测了一个基因的序列，测通之后想把它拼起来，先用Vector NTI拼，没拼成功，出现很多非ATGC字符，后来尝试用DNAstar，却无法拼成一条序列，显示有两个contigs。不知哪位兄弟用过DNAstar的这个功能，帮忙看看。
愿以威望值相赠。 我已经给你拼好了。
序列是人蛋白精氨酸甲基转移化酶的反义链。
直接拼接得到的序列：
>Contig_1
CCAGCTATTTAGGTGACACTATAGAATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGC
CGCCAGTGTGCTGGAATTCGGCTTTATTTACTCGAGGCTAGAGGCCAATGGTATATGAGCGGCCTGTGGGGTTATGAAT
AGCAGAACAGACTGGTGCTGTCACAGCCCACTCATACCACACCTTCTTGGAATTGCTGCATCGCCAGAAACGCACACAG
ATGGTTTGGCCTTCACGTACCGTTATGGGCTGCTTAATAGGGAAGAGGATGGGAAACCATGAGAACATCCCAGGAGAGT
GAGTCTCTGGACGGATACTCAGAGTGATGTCCTGATAAAGCACAGTCTCAAAGTAGCCGGCAAAGCCATGTAGTACTGT
GTTCACCTCCACAGGAAATTCCAAGGTGCAATAGCGGTTGTTGTCAATCATAGGATCTCTGTTGGGATGGCTGAAGGTG
AAACAGGGCTGGGGTGCAGAGAGCTGGTGGAAGTTGTGCGGCCGTACCACATAAGGCATCTCAAACTGGGCCTCAGGGT
CACGGTCCTTCTCCCTACAGGCTCGGACCTCATTGTACAGCTTGGAGGAAGAGATGGGAGCCAGAAAGGAAGTGTACTC
CCCGGGGATGCTCACACCATCATCTTTTAGGAAGTGCTGGGCTCCATCCAGGCACTCAGGCGACAATTCATTGTCAGCA
AATGAGCCCAAAAGCTCACTGACAATGATGTCTGCTTTCTCTGGAGCCACCCATTCCCTCATGTCTGATGAGACTACGG
TCACTTGGCTTCCCCATTCTTCAAACTGCCAGTTCTCTAGCGTCACCGCGGCATTTGGGTTTTTCTCCACAGCATACAG
CTTTATCCGCCGGTCGGCCTGCTTGGCTGCCCGCAGGGAAGCGTTCACCAGGGGTCCCCGTCCTGCTCCCAGCACCATC
AGTACCTGGACATTGGTATCCTTCTCCTCTTCTGGTACTCGGTCTAGCAGACATTTATAGATGGCCTGCTGGTACTGAG
AGTATTTGATGGGGTCCTTTTCAAACACTTCATATGTCTGAGATTCCAGATTGTCCATCAGTGGCTGAAGCGGGGACTG
CAGATAGTCTTCATAGCCCTTGGCAAAGAGTTCATAGGCATTAGGTGGAGGACGGTTCTGGCTTAAGTACTCCAGGTAT
TGGAGGTAGGAGCAGAACTCCTTCTCTGAGTGGTGGTTGGTGCCTGTGATGATGAACTGCACCTCCAACTTGAGGAGCC
GGAAGATGAGCCTCTGGTGCATCTTAGAAAGAACAGGAAATCCCTTCTTATTGGTCAGGAAAATGCTAGTGGGGAGAAT
GGCTGCTTTGATGGGCTCCCCAAGCCAGCGATCAGTGACATGATTAGATGGGAGGTCAGCCCCAATTTCAAGAGCCACT
GCAATCCTCTTACTATAGTCACACAAAGTCCGGAAGTTGTGCCACCACATCCACGTTTTCTCCTCCCCACTGTACTCCT
CTGTGTGTGTAGTTGGTGCATTCTCAATTATATCATCTCCCAGGTCCTCTGGTGCCACCAAGGGTACCCGCATCCAGAA
CATGGAAGAGTGATGGCCAGTGTGGATGTGGTTGGTCAAAACTCTGGCCAGGTTGGTGTTATCTTCCTGATTAAGGGGC
AGCAGGAAAGCTGGAAGACCCAAATATGCACCAAAATTCAGCTCCTGTAACATGGCCGCCTCGGAGTTCCTGCGAATCT
TCTCCACTTTTGAGTCTGGACGAATCCATGGAGAAAGCTTTCCCACAATTAGCGTATTCCAGTCCCTTCCTGACAGCAG
TAGGTCTGATCGTGTCTGGGGACCGGGCCGATTCTTAGCAGGTTCCTGAATGAACTCCCTCTTGAAACGCGGATGGAAG
ACAGGCATGCAGAGGAAATCAAACCCCTGCTTGGCCACAGCCCCTAGTGTGTCAGCTATTTCGGGGACGCAATTCAGGT
CCCTCCCGCTGGACACGCGGCTCCCACCAGCACCCCCGACCGCCATCGCCGCCATCTTTCTCCTCGCGCTGTCCACGGA
TCCATTTATAAGCCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATTCGCC
CTATAGTGAGTCGTATACAATCACTGCCG
互补反转后得到的对应的正义链的序列：
>ContigRevComp
CGGCAGTGATTGTATACGACTCACTATAGGGCGAATTGGGCCCTCTAGATGCATGCTCGA
GCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGGCTTATAAATGGATCCGTGGACAGC
GCGAGGAGAAAGATGGCGGCGATGGCGGTCGGGGGTGCTGGTGGGAGCCGCGTGTCCAGC
GGGAGGGACCTGAATTGCGTCCCCGAAATAGCTGACACACTAGGGGCTGTGGCCAAGCAG
GGGTTTGATTTCCTCTGCATGCCTGTCTTCCATCCGCGTTTCAAGAGGGAGTTCATTCAG
GAACCTGCTAAGAATCGGCCCGGTCCCCAGACACGATCAGACCTACTGCTGTCAGGAAGG
GACTGGAATACGCTAATTGTGGGAAAGCTTTCTCCATGGATTCGTCCAGACTCAAAAGTG
GAGAAGATTCGCAGGAACTCCGAGGCGGCCATGTTACAGGAGCTGAATTTTGGTGCATAT
TTGGGTCTTCCAGCTTTCCTGCTGCCCCTTAATCAGGAAGATAACACCAACCTGGCCAGA
GTTTTGACCAACCACATCCACACTGGCCATCACTCTTCCATGTTCTGGATGCGGGTACCC
TTGGTGGCACCAGAGGACCTGGGAGATGATATAATTGAGAATGCACCAACTACACACACA
GAGGAGTACAGTGGGGAGGAGAAAACGTGGATGTGGTGGCACAACTTCCGGACTTTGTGT
GACTATAGTAAGAGGATTGCAGTGGCTCTTGAAATTGGGGCTGACCTCCCATCTAATCAT
GTCACTGATCGCTGGCTTGGGGAGCCCATCAAAGCAGCCATTCTCCCCACTAGCATTTTC
CTGACCAATAAGAAGGGATTTCCTGTTCTTTCTAAGATGCACCAGAGGCTCATCTTCCGG
CTCCTCAAGTTGGAGGTGCAGTTCATCATCACAGGCACCAACCACCACTCAGAGAAGGAG
TTCTGCTCCTACCTCCAATACCTGGAGTACTTAAGCCAGAACCGTCCTCCACCTAATGCC
TATGAACTCTTTGCCAAGGGCTATGAAGACTATCTGCAGTCCCCGCTTCAGCCACTGATG
GACAATCTGGAATCTCAGACATATGAAGTGTTTGAAAAGGACCCCATCAAATACTCTCAG
TACCAGCAGGCCATCTATAAATGTCTGCTAGACCGAGTACCAGAAGAGGAGAAGGATACC
AATGTCCAGGTACTGATGGTGCTGGGAGCAGGACGGGGACCCCTGGTGAACGCTTCCCTG
CGGGCAGCCAAGCAGGCCGACCGGCGGATAAAGCTGTATGCTGTGGAGAAAAACCCAAAT
GCCGCGGTGACGCTAGAGAACTGGCAGTTTGAAGAATGGGGAAGCCAAGTGACCGTAGTC
TCATCAGACATGAGGGAATGGGTGGCTCCAGAGAAAGCAGACATCATTGTCAGTGAGCTT
TTGGGCTCATTTGCTGACAATGAATTGTCGCCTGAGTGCCTGGATGGAGCCCAGCACTTC
CTAAAAGATGATGGTGTGAGCATCCCCGGGGAGTACACTTCCTTTCTGGCTCCCATCTCT
TCCTCCAAGCTGTACAATGAGGTCCGAGCCTGTAGGGAGAAGGACCGTGACCCTGAGGCC
CAGTTTGAGATGCCTTATGTGGTACGGCCGCACAACTTCCACCAGCTCTCTGCACCCCAG
CCCTGTTTCACCTTCAGCCATCCCAACAGAGATCCTATGATTGACAACAACCGCTATTGC
ACCTTGGAATTTCCTGTGGAGGTGAACACAGTACTACATGGCTTTGCCGGCTACTTTGAG
ACTGTGCTTTATCAGGACATCACTCTGAGTATCCGTCCAGAGACTCACTCTCCTGGGATG
TTCTCATGGTTTCCCATCCTCTTCCCTATTAAGCAGCCCATAACGGTACGTGAAGGCCAA
ACCATCTGTGTGCGTTTCTGGCGATGCAGCAATTCCAAGAAGGTGTGGTATGAGTGGGCT
GTGACAGCACCAGTCTGTTCTGCTATTCATAACCCCACAGGCCGCTCATATACCATTGGC
CTCTAGCCTCGAGTAAATAAAGCCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATC
CGAGCTCGGTACCAAGCTTGATGCATAGCTTGAGTATTCTATAGTGTCACCTAAATAGCT
GG
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