Contents
Isolation of DNA fragments using glass milk (GENE-CLEAN)
SOUTHERN BLOT
SOUTHERN ANALYSIS OF MOUSE TOE/TAIL DNA
Northern transfer protocol
Hybridizing Northern blots with riboprobes
cDNA + GENOMIC DNA LIBRARY SCREENING
Isolation of Mouse genomic DNA
RNA ISOLATION USING GUHCL
POLY A+ RNA PREPARATION using proteinase K and SDS
SELECTION OF POLYADENYLATED RNA FROM TOTAL RNA
Mini plasmid preps
Large Scale Plasmid Preps
Preparing and USING bacterial cells for electroporation
In situ hybridisation to RNA in tissue sections
PRIMER LABELLING FOR PRIMER EXTENSION ASSAYand PRIMER EXTENSION
DNA-se I FOOTPRINTING
Double stranded DNA sequencing (Dideoxy)
Buffer gradient SEQUENCING GELS
Nested Deletions using exonuclease-III and mung bean nuclease
SITE DIRECTED MUTAGENESIS USING THE UNG - DUT SYSTEM.
In vitro mutagenesis using Altered Sites
Labeling of oligonucleotides
RANDOM HEXAMER LABELLING OF CDNA PROBES
RNAse protection assay
Isolation of phage DNA
PHAGE PURIFICATION WITH DE52
BUTANOL PRECIPITATION OF DEPROTECTED OLIGONUCLEOTIDES
OLIGO PURIFICATION on acrylamide gels
POLYMERASE CHAIN REACTION
Rapid Amplification of mRNA ENDS by PCR (RACE)
YEAST MEDIA
TRANSFORMATION OF YEAST
Modified yeast transformation
Isolation of shuttle vectors from yeast and transformation into E. coli
Yeast lysates for westerns
地址
http://wheat.pw.usda.gov/%7Elazo/methods/au/molbiol.html
