Oxford Practical Approach Series 5本蛋白质相关 Approach, Oxford, 蛋白质, Series, USA

cytokines

1.Protein Purification Techniques: A Practical Approach

Publisher:  Oxford University Press, USA
Number Of Pages:  280
Publication Date:  2001-03-12
Sales Rank:  341440
ISBN / ASIN:  0199636737
EAN:  9780199636730
Binding:  Paperback
Manufacturer:  Oxford University Press, USA
Studio:  Oxford University Press, USA
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Book Description:

Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

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2.Protein Phosphorylation


Protein Phosphorylation: A Practical Approach (Practical Approach Series)

Publisher:  Oxford University Press, USA
Number Of Pages:  431
Publication Date:  1999-12-15
Sales Rank:  1369361
ISBN / ASIN:  0199637288
EAN:  9780199637287
Binding:  Paperback
Manufacturer:  Oxford University Press, USA
Studio:  Oxford University Press, USA


Book Description:
Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.

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3.Protein Expression


Protein Expression: A Practical Approach (Practical Approach Series)

Publisher:  Oxford University Press, USA
Number Of Pages:  304
Publication Date:  1999-06-30
Sales Rank:  1575362
ISBN / ASIN:  0199636230
EAN:  9780199636235
Binding:  Paperback
Manufacturer:  Oxford University Press, USA
Studio:  Oxford University Press, USA

Book Description:
Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.


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4.Gel Electrophoresis of Proteins


Gel Electrophoresis of Proteins: A Practical Approach (Practical Approach Series)

Publisher:  Oxford University Press, USA
Number Of Pages:  376
Publication Date:  1998-11-11
Sales Rank:  1064252
ISBN / ASIN:  0199636400
EAN:  9780199636402
Binding:  Paperback
Manufacturer:  Oxford University Press, USA
Studio:  Oxford University Press, USA

Book Description:
This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods were presented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.


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5.High Resolution Chromatography


High Resolution Chromatography: A Practical Approach (Practical Approach Series)

Publisher:  Oxford University Press, USA
Number Of Pages:  336
Publication Date:  1999-06-21
Sales Rank:  5090028
ISBN / ASIN:  0199636494
EAN:  9780199636495
Binding:  Hardcover
Manufacturer:  Oxford University Press, USA
Studio:  Oxford University Press, USA

Book Description:
The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.

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