tianjiaoya

我要克隆一个4、5百k的基因，但是由于地处边疆，实验室条件十分简陋，各种仪器都需要到处去借用，很难连贯的进行试验操作，结果克隆了半年了，没有克隆出来，我的DNA模板和引物肯定是没有问题的，这段基因也不是什么很特殊的基因，没有特殊结构和高GC含量，哪位大侠能帮我扩增一下啊？连到T载上就行。我可以提供酶、DNA模板、引物等试验所需一切药品，呵呵，当然，扩增完成后，会有酬谢的，送试剂或者money都可以，着急啊，请尽快联系我啊，在下面回贴留下联系方式，或tianjiaoya@163.com 。

saturn100

i suggest you to change a lab to finish you work

hehe

tianjiaoya

to:saturn100
    I think that  your suggestion  is very good. But there are many difficulties. For example, there is no lab I can jion in. And I have not enough money to support it. My boss does not allow me to go out to finish my work. Because it will spend many money.
    All in all, I have to finish my work in this lab. But there is no enough equipment for me to finish my experiment. So I want to find help. Who can help me? Please sent an e-mail to my e-mail box or call me. My mobile phone number is 13009626439. Thank you for your help!

wangxuexia

同情你啊！！！！！！